| 16/03/2001 | 18:00 | Stuttgarter Kickers | - | Hannover 96 | 2:0 (1:0) | |
| 18:00 | Alemannia Aachen | - | 1. FC Saarbrücken | 1:0 (1:0) | ||
| 17/03/2001 | 14:00 | 1. FC Nürnberg | - | SpVgg Greuther Fürth | 0:1 (0:1) | |
| 14:00 | Rot-Weiß Oberhausen | - | SSV Ulm 1846 | 3:1 (2:1) | ||
| 18/03/2001 | 14:00 | FC St. Pauli | - | Chemnitzer FC | 3:0 (1:0) | |
| 14:00 | SSV Reutlingen | - | MSV Duisburg | 0:1 (0:1) | ||
| 14:00 | 1. FSV Mainz 05 | - | VfL Osnabrück | 2:0 (1:0) | ||
| 14:00 | LR Ahlen | - | Bor. Mönchengladbach | 2:1 (2:1) | ||
| 19/03/2001 | 19:15 | Waldhof Mannheim | - | Arminia Bielefeld | 3:3 (1:2) |
| » Tables | Home | Away | First round | Second round | First half | Second half |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
1. FC Nürnberg | 26 | 17 | 4 | 5 | 50:26 | 24 | 55 |
| 2 |
|
Bor. Mönchengladbach | 26 | 13 | 9 | 4 | 50:25 | 25 | 48 |
| 3 |
|
FC St. Pauli | 26 | 13 | 7 | 6 | 54:41 | 13 | 46 |
| 4 |
|
SpVgg Greuther Fürth | 26 | 12 | 7 | 7 | 41:27 | 14 | 43 |
| 5 |
|
Waldhof Mannheim | 26 | 12 | 7 | 7 | 40:34 | 6 | 43 |
| 6 |
|
Rot-Weiß Oberhausen | 26 | 12 | 5 | 9 | 37:33 | 4 | 41 |
| 7 |
|
SSV Reutlingen | 26 | 11 | 6 | 9 | 50:42 | 8 | 39 |
| 8 |
|
MSV Duisburg | 26 | 11 | 5 | 10 | 40:31 | 9 | 38 |
| 9 |
|
LR Ahlen | 26 | 10 | 8 | 8 | 42:41 | 1 | 38 |
| 10 |
|
Hannover 96 | 26 | 10 | 7 | 9 | 40:31 | 9 | 37 |
| 11 |
|
Alemannia Aachen | 26 | 10 | 6 | 10 | 37:49 | -12 | 36 |
| 12 |
|
1. FC Saarbrücken | 26 | 9 | 7 | 10 | 36:49 | -13 | 34 |
| 13 |
|
1. FSV Mainz 05 | 26 | 7 | 8 | 11 | 27:34 | -7 | 29 |
| 14 |
|
Arminia Bielefeld | 26 | 6 | 9 | 11 | 37:36 | 1 | 27 |
| 15 |
|
Stuttgarter Kickers | 26 | 6 | 9 | 11 | 24:35 | -11 | 27 |
| 16 |
|
SSV Ulm 1846 | 26 | 6 | 7 | 13 | 31:40 | -9 | 25 |
| 17 |
|
VfL Osnabrück | 26 | 5 | 9 | 12 | 23:40 | -17 | 24 |
| 18 |
|
Chemnitzer FC | 26 | 2 | 4 | 20 | 19:64 | -45 | 10 |