| Micah Abbey | Donatello (voice) | |
| Shamon Brown Jr. | Michelangelo (voice) | |
| Nicolas Cantu | Leonardo (voice) | Die Fabelmans, The Walking Dead: World Beyond, Tales of the Teenage Mutant Ninja Turtles |
| Brady Noon | Raphael (voice) | Family Switch, The Mighty Ducks: Game Changers, Tales of the Teenage Mutant Ninja Turtles |
| Ayo Edebiri | April O'Neil (voice) | |
| Maya Rudolph | Cynthia Utrom (voice) | CHiPS, IF: Imaginäre Freunde, Big Mouth, Loot, Big Hero 6: The Series, Up All Night, Bless the Harts, Human Resources, Forever (2018), The Second Best Hospital in the Galaxy, The Hospital, The Spoils Before Dying, Brothers in Atlanta |
| John Cena | Rocksteady (voice) | The Suicide Squad, F9: The Fast Saga, Fast & Furious 10, Barbie, Argylle, Die Hart 2: Die Harter, Peacemaker |
| Seth Rogen | Bebop (voice) | Kung Fu Panda 3, Der Super Mario Bros. Film, Die Fabelmans, Dumb Money - Schnelles Geld, Mufasa: Der König der Löwen, Invincible, Freaks and Geeks, Pam & Tommy, The Studio, Sausage Party: Foodtopia, Platonic, Undeclared, Santa Inc. |
| Rose Byrne | Leatherhead (voice) | Insidious 5, Ezra – Eine Familiengeschichte, Damages, Physical, Mrs. America, Platonic |
| Natasia Demetriou | Wingnut (voice) | |
| Giancarlo Esposito | Baxter Stockman (voice) | Megalopolis, Abigail, MaXXXine, The Electric State, Captain America: Brave New World, The Mandalorian, Better Call Saul, Godfather of Harlem, The Gentlemen, Harley Quinn, Homicide: Life on the Street, Revolution, Creepshow, Parish, Jett, The Get Down, Rebel, South Beach |
| Jackie Chan | Splinter (voice) | Kung Fu Panda 3, Karate Kid: Legends |
| Ice Cube | Superfly (voice) | |
| Paul Rudd | Mondo Gecko (voice) | Ghostbusters: Legacy, Ant-Man and the Wasp, Ant-Man and the Wasp: Quantumania, Ghostbusters: Frozen Empire, Only Murders in the Building, What If...?, The Shrink Next Door, Wet Hot American Summer, Living with Yourself |
| Post Malone | Ray Fillet (voice) | |
| Hannibal Buress | Genghis Frog (voice) | |
| MrBeast | Times Square Guy (voice) | |
| Derek Wilson | Spider (voice) | |
| Lukas Williams | Curious Goon (voice) | |
| Michael Badalucco | Bad Bernie (voice) | |
| Dempsey Pappion | Bald Bronson (voice) | |
| John Capodice | Cab Driver (voice) | |
| Andia Winslow | TV Anchor (voice) | |
| Raechel Wong | News Anchor (voice) | |
| David Faustino | Normal Nate (voice) | |
| Danny Mastrogiorgio | Toupee Tom / Police Officer (voice) | |
| Noel Gibson | Responding Police Officer / Military Advisor (voice) | |
| Myra Owyang | Short Sharon (voice) | |
| Kevin Eastman | Good Human (voice) | |
| Alex Hirsch | Bossy Goon / Scumbug (voice) | |