Fig. 2

Differential gene expression in 10w and 10d mdx and C57Bl/10 cerebra. A Venn diagram showing the number of genes differentially expressed (FDR 10%) between mdx and C57BL/10 mice. The largest ellipse contains all of the genes regulated by genotype (both ages considered together). The smaller circles show genes that were also significantly altered in pairwise comparisons between mdx and C57BL/10 mice at each age. B Heatmap showing all genes regulated at an FDR < 10% according to the genotype factor. The top 10 genes within each cluster and the lowest genotype FDR are marked for clarity. The intensity of the coloured rectangles represents transcript abundance levels. The presented level is proportional to the row z score values (between darkest blue: −3 and darkest red: 3), as displayed on the bar above the heatmap image. Hierarchical clustering was performed using correlation as a distance measure. The light and dark orange bars highlight whether the gene is differentially expressed (FDR < 10%) in 10d and 10w mdx cerebra compared to those in the cerebra of age-matched controls. A full list is available in Supplementary Table 1. C Profiles of differences for the four identified clusters of altered genes; mean transcript abundance levels are presented for each group of genes