| 1st(1st leg) | RB Omiya Ardija | - | Yokohama F. Marinos | 2:1 (1:0) | ||
| 2nd (2nd leg) | Yokohama F. Marinos | - | RB Omiya Ardija | 1:0 (1:0) | ||
| 1st(1st leg): 31/08/2016 11:00 | 2nd (2nd leg): 04/09/2016 10:00 | Winner: Yokohama F. Marinos | ||||
| 1st(1st leg) | Sanfrecce Hiroshima | - | Gamba Osaka | 1:1 (1:0) | ||
| 2nd (2nd leg) | Gamba Osaka | - | Sanfrecce Hiroshima | 6:3 (2:1) | ||
| 1st(1st leg): 31/08/2016 11:00 | 2nd (2nd leg): 04/09/2016 11:00 | Winner: Gamba Osaka | ||||
| 1st(1st leg) | Vissel Kobe | - | Urawa Red Diamonds | 1:2 (1:2) | ||
| 2nd (2nd leg) | Urawa Red Diamonds | - | Vissel Kobe | 4:0 (2:0) | ||
| 1st(1st leg): 31/08/2016 11:00 | 2nd (2nd leg): 04/09/2016 10:30 | Winner: Urawa Red Diamonds | ||||
| 1st(1st leg) | FC Tokyo | - | Avispa Fukuoka | 1:1 (0:0) | ||
| 2nd (2nd leg) | Avispa Fukuoka | - | FC Tokyo | 0:2 (0:1) | ||
| 1st(1st leg): 31/08/2016 11:30 | 2nd (2nd leg): 04/09/2016 11:00 | Winner: FC Tokyo | ||||