Papers by Sueli Van Der Sand
Investigação das comunidades bacterianas cultiváveis e aspectos físico-químicos em wetlands construídos no Hotel SPA do Vinho
ATIVIDADE ANTIFUNGICA DE Bacillus E 164 CONTRA Bipolaris sorokiniana
Biociências (On-line), Mar 3, 2010
Spot blotch caused by Bipolaris sorokiniana is an important disease of wheat in warmer and humid ... more Spot blotch caused by Bipolaris sorokiniana is an important disease of wheat in warmer and humid weather conditions, whose control relies mainly on chemical antifungal agents. The purpose of this work were to isolate bacteria strains with antifungal activity against B. sorokiniana; to select the best inhibitor and to evaluate the best growth conditions for the antifungal production; and to test it´s action in vivo. The bacterial strains were pre-screened against four B. sorokiniana isolates on plates containing Sabouraud maltose agar. The isolate that showed the best result was grown on different culture media, cells were filtrated and the filtrates were tested against B.sorokiniana on plates with PDA medium. The in vivo test was done on wheat seeds, infected with B. sorokiniana isolate on a chamber with controlled temperature. Bacillus E164 was chosen among the 86 bacterial isolates tested against the phytopathogen. The filtrate from Bacillus E164 grown on tryptic casein soy broth (TSB) and straw culture media showed a similar degree of inhibition against the phytopathogen, the same result was not observed with malt extract broth media. The filtrate culture showed activity similar to control being submitted to temperatures from 50- 90oC. The in vivo test with isolate E164 caused morphological effects on wheat plants with a significant root length reduction. Control exerted by Bacillus E164 over B. sorokiniana isolates was relevant in vitro, nevertheless the influence and importance of the metabolites produced must be elucidated for the application in vivo.
Composting is a natural biological process that degrades organic matter which is carried out spon... more Composting is a natural biological process that degrades organic matter which is carried out spontaneously by aerobic microorganisms, whose metabolic action leads to the mineralization and partial humification of organic residue. The aim of this study was to investigate survival of potentially pathogenic bacterial species throughout the process and detection of enterotoxigenic Escherichia coli (ETEC) in the compost using PCR. The presence of heterotrophic bacteria, total total and fecal coliforms, and bacteria succession were also determined. Out of the total 222 bacterial colonies isolated, 33 different genera and 56 different species were identified, being Bacillus, Escherichia, Enterobacter, and Pseudomonas, the prevailing genera. Total coliform counts ranged between 9.0 x 10 4 and 3.0 x 10 6 cfu/g, and fecal coliform counts between 1.0 x 10 4 and 2.3 x 10 6 cfu/g. The composting process was partially efficient, since even though coliform counts were reduced, enterotoxigenic E. coli was detected in mature compost.
Acta Scientiae Veterinariae, Jun 27, 2018
As lagoas de estabilização para tratamento de esgoto sanitário são processos biológicos eficiente... more As lagoas de estabilização para tratamento de esgoto sanitário são processos biológicos eficientes e econômicos utilizados como solução satisfatória para a remoção de microrganismos potencialmente patogênicos. Neste trabalho, foram isoladas e identificadas bactérias aeróbias e anaeróbias facultativas presentes na Estação de Tratamento de Esgoto (ETE)-Ipanema, Porto Alegre/RS com o objetivo de avaliar a eficiência do processo de tratamento de esgotos. Foram realizadas coletas trimestrais durante o período de um ano. As amostras foram coletadas do afluente, da lagoa facultativa, da lagoa de maturação e do efluente, semeadas em diferentes meios de cultura para a identificação bacteriana. Foram isoladas e identificadas 392 bactérias, destas 40.8% pertencentes ao gênero Enterobacter, 29.6% ao gênero Bacillus, 6.63% de Acinetobacter e, 4.84% do gênero Alcaligenes e os demais 18.13% distribuídos entre outros 12 gêneros, os quais foram predominantemente Gram-negativos. Observou-se um decréscimo no número de coliformes totais e fecais no afluente e efluente, como dos gêneros bacterianos durante as etapas do sistema de tratamento bem como a DBO, demonstrando a eficácia do processo. Descritores: microrganismos patogênicos, tratamento de esgotos, lagoas de estabilização, coliformes totais e fecais.
Universidade Federal do Rio Grande do Sul –UFRGS. E-mail : thaifeltrin@gmail.com 2 Doutorandas do... more Universidade Federal do Rio Grande do Sul –UFRGS. E-mail : thaifeltrin@gmail.com 2 Doutorandas do PPG de Microbiologia Agrícola e do Ambiente , Universidade Federal do Rio Grande do Sul – UFRGS 3 Mestrandado PPG de Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul – UFRGS. 3 Mestranda do PPG de Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul – UFRGS. 4 Aluna de Graduação em Biomedicina, Universidade Federal do Rio Grande do SulUFRGS. 5 Pro. Assistente III, Departamento de Microbiologia, Imunologia e Parasiotologia, Coordenadora do Grupo de Pesquisa, Universidade Federal do Rio Grande do Sul – UFRGS. ISSN 2237-1672 V SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA APLICADA
Investigação da atividade antibacteriana dos basidiomicetos Lentinula boryana e Lentinula edodes
Two species of basidiomycetes, Lentinula boryana and Lentinula edodes, were evaluated for their a... more Two species of basidiomycetes, Lentinula boryana and Lentinula edodes, were evaluated for their antibacterial activities, biomass production and growth in two different culture media. The basidiomycete L. boryana occurs naturally in the Brazilian territory whilst L. edodes grows in Asia. Mycelia from each species were incubated in liquid media for 28 days and vacuum-filtered. L. boryana showed the largest biomass production in both culture media when compared to L. edodes, which presented significant differences in growth when cultivated in different culture media. Antibacterial activity of the two species was evaluated against 10 bacterial species, six of them being of clinical importance. Both basidiomycetes L. boryana and L. edodes showed antibacterial activity against B. cereus and S. aureus, although only L. edodes was active against S. mutans. Key words: Basidiomycetes, Lentinula boryana, Lentinula edodes (shiitake mushroom), antibacterial activity.
Uma forma de se controlar a contaminação de um corpo d`água por microrganismos patogênicos é atra... more Uma forma de se controlar a contaminação de um corpo d`água por microrganismos patogênicos é através de sua remoção na etapa do tratamento de esgotos. Este projeto propõe avaliar a eficiência na remoção dos microrganismos potencialmente patogênicos da Estação de Tratamento de Esgotos Ipanema (ETE-DMAE). A ETE realiza o tratamento do esgoto através de lagoas de estabilização em série, tendo numa 1ª etapa 01 Lagoa Anaeróbia, 02 Lagoas Facultativas e 03 Lagoas de Maturação, com um tempo de detenção de 9 dias e com uma eficiência estimada de 99,89% na remoção de microrganismos indicadores da contaminação fecal (Grupo Coliforme). Para o projeto, foram coletadas amostras trimestrais do esgoto bruto (afluente), da lagoa facultativa, da lagoa de maturação e do efluente no período de julho/97 à junho/98. As amostras foram diluídas em água peptonada e semeadas nos meios de cultura seletivos para as espécies Salmonella sp., Yersínia sp., Vibrio cholerae, Campylobacter jejuni, Escherichia coli e Shigella sp. Após o período de incubação de 24-48h foram selecionadas colônias características de cada meio e identificadas através de testes bioquímicos. Os resultados preliminares mostram uma baixa concentração de microrganismos potencialmente patogênicos presentes nas amostras. Também evidencia-se uma redução na remoção de bactérias dos gêneros Enterobacter, Proteus e Pseudomonas, cujo tratamento apresentou-se satisfatório na remoção de organismos (Fapergs, Cnpq, Capes)
Potencial enzimático de actinomicetos utilizados para controle biológico de Bipolaris sorokiniana
A contaminação de um corpo d`água por microrganismos patogênicos ocorre através do despejo de mat... more A contaminação de um corpo d`água por microrganismos patogênicos ocorre através do despejo de material fecal de animais de sangue quente, tornando-se um risco à saúde pública. Tendo em vista estes aspectos, o trabalho tem como objetivo isolar e identificar bactérias potencialmente patogênicas presentes na ETE de Ipanema-Porto Alegre, que utiliza o sistema de lagoas de estabilização. As coletas das amostras foram realizadas trimestralmente no período de julho/97 a julho/98, sendo estas do afluente, da lagoa facultativa, da lagoa de maturação, do efluente e de dois pontos ajusante e montante do lançamento do efluente. As amostras foram diluídas em água peptonada e semeadas no meio de cultivo Agar Base Sangue de Ovino e nos meios seletivos para Yersínia, Salmonella-Shigella, Pseudomonas, EMB, TCBS, Ágar SKIRROW, XLT4 e XLD. Após o período de incubação de 24-48h é feita a contagem de colônias presentes nos respectivos meios, sendo estas selecionadas aleatoriamente de cada meio e identificadas através da coloração de gram e de testes bioquímicos. Até o presente momento foram isoladas 415 colônias de bactérias, sendo que destas, 170 colônias foram identificadas e classificadas como pertencentes aos Gêneros Enterobacter (39%), Pseudomonas (20%), Proteus (19,7%), Escherichia (6,0%), Citrobacter (5,9%), Aeromonas (5,3%), Serratia (4,1%). Os resultados preliminares não indicam a presença significativa de microrganismos patogênicos presentes nas amostras e os demais isolamentos e identificações encontram-se em andamento (FAPERGS, CAPES, CNPq).
Ambiente/UFRGS; elisminotto@yahoo.com.br 2 Esudante de biomedicina, bolsista de iniciação científ... more Ambiente/UFRGS; elisminotto@yahoo.com.br 2 Esudante de biomedicina, bolsista de iniciação científica CNPq do laboratório de micologia, ICBS, UFRGS; 3Biomédica, FEEVAlE; estagiária do laboratório de micologia, ICBS, UFRGS; 5 Bióloga, mestranda (CAPES) do Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente/UFRGS; 6 Profa Dra. Orientadora e Coordenadora do Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente/UFRGS; ISSN 2237-1672 V SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA APLICADA
DMAE 1). O meio ambiente é a fonte de todos os recursos naturais vitais para a manutenção e desen... more DMAE 1). O meio ambiente é a fonte de todos os recursos naturais vitais para a manutenção e desenvolvimento sócio econômico da civilização humana. Dentre estes recursos destaca-se a água, um recurso renovável em quantidade porém esgotável em qualidade. Tendo em vista este aspecto, o trabalho tem como objetivo isolar e identificar as bactérias presente no baalneário de Belém novo para desta forma poder verificar a qualidade de suas águas. As coletas foram realizadas mensalmente no período de abril de 1999 a maio de 2000. As amostras foram diluídas em água peptonada e semeadas em ágar nutriente. Após o período de incubação de 24-48 h foram selecionadas colônias isoladas que posteriormente foram identificadas através da coloração de Gram e testes bioquímicos. Até o presente momento foram isoladas e identificadas 332 colônias de bactérias. Estas foram classificadas como pertencentes aos gêneros Bacillus (56,9%), Enterobacter (12,1%), Staphylococcus (10,5%), Escherichia coli (7%), Corynebacterium (4,8%), demais gêneros (8,8%). Este trabalho terá prosseguimento com testes adicionais para classificar as bactérias encontradas até o nível de espécie.
Anais da Academia Brasileira de Ciências, 2018
This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms iso... more This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms isolated from a biological treatment system of effluents from a hotel. To select the best lipolytic microorganism for use in biotechnological processes, we tested 45 bacterial isolates recovered from the raw effluent of the hotel's restaurant waste tank. Lipase production was assayed in culture medium supplemented with olive oil and rhodamine B, incubated at 25 °C and 30 °C for 24 h-48 h. Results showed 22 isolates lipase producers. All isolates were inoculated on medium without yeast extract to select the ones with highest enzyme yields. Out of these, nine isolates showed high lipase activity. The strain with the larger halo was assayed in submerged culture using an orbital shaker and a bioreactor, with three different substrates (olive oil, grape seed oil, and canola oil). Isolate G40 identified as Acinetobacter baylyi was selected to run the production assays because it showed the best result in the solid medium. In the bioreactor, maximum lipase production was obtained after 12 h of culture with the three substrates evaluated: 0,358 U/mL.min-1 in olive oil, 0,352 U/mL.min-1 with grapeseed oil, and 0,348 U/mL.min-1 with canola oil.
Journal of agricultural science, Nov 15, 2017
Streptomyces sp. R18(6) and Streptomyces sp. 6(4) strains were evaluated for their ability to con... more Streptomyces sp. R18(6) and Streptomyces sp. 6(4) strains were evaluated for their ability to control brown spot and common root rot caused by Bipolaris sorokiniana in wheat crops. The antifungal activity of these isolates was tested using a double-layer assay and culture pairing at 28 °C. Physiological and enzymatic activity performed through siderophore, indole-3-acetic acid, nitrogen fixation and phosphate solubilization tests. The biocontrol of the disease and growth-promoting efficiency of wheat seedlings were assessed using in vivo assays in a greenhouse. In the culture pairing assays, both strains inhibited B. sorokiniana mycelial growth, while in the double-layer only Streptomyces sp. R18(6). Streptomyces sp. 6(4) produced auxin, siderophores, fixed nitrogen and solubilized phosphate, whereas R18(6) did not produce siderophores. In the greenhouse assays, strain R18(6) showed statistical differences in shoot dry mass and root dry mass compared with those of strain 6(4) in the presence of the phytopathogen (P ≤ 0.05), and these results were more evident when the environmental temperature was higher. In the absence of the phytopathogen, Streptomyces sp. 6(4) strain increased the root dry mass compared with that of the control during the same period. Therefore, these isolates can potentially control root rot and brown spotting and may promote the growth of wheat plants.
The intricate molecular identification of Streptomyces: a case study on Antarctic soil isolates
Archives of Microbiology, Jul 13, 2022
Whole-genome sequencing-based characterization of Streptomyces sp. 6(4): focus on natural product
Access microbiology, Mar 1, 2023
We have sequenced the whole genome of Streptomyces sp. 6(4) isolated from tomato roots that prese... more We have sequenced the whole genome of Streptomyces sp. 6(4) isolated from tomato roots that presents antifungal activity against phytopathogenic fungi, mainly Bipolaris sorokiniana. The genome has almost 7 Mb and 3368 hypothetical proteins that were analysed and characterized in Uniprot with the emphasis on biological compounds. Multilocus sequence typing (MLST) analyses were performed in an effort to characterize and identify this isolate, resulting in a new sequence type (ST), classified as ST64. Phenetic and phylogenetic trees were constructed to investigate Streptomyces sp. 6(4) evolution and sequence similarity, and the isolate is a strain closer to Streptomyces prasinus and Streptomyces viridosporus . It is known that the genus Streptomyces possess huge metabolic capacity with the presence of cryptic genes. These genes are usually present in clusters, which are responsible for the production of diverse natural products, mainly antibiotics. In addition, 6(4) showed 11 biosynthetic gene clusters through antiSMASH, including 3 polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) type clusters.
Avaliação Da Diversidade De Isolados De Actinobactérias Utilizando Box-PCR e Urp-PCR
Uso da técnica de rep-PCR na identificação de isolados de actinomicetos
Occurrence and susceptibility of yeasts present in the polluted waters of Dilúvio Stream, Porto Alegre, Brazil
Revista Brasileira de Biociências, 2019
Water is one of the naturally occurring essential requirements of all life functions. Today wate... more Water is one of the naturally occurring essential requirements of all life functions. Today water pollution has become one of the major problems for all life forms. Diluvio Stream is one of the main watercourses in Porto Alegre, Brazil and receives domestic and hospital sewage and rain waters, which favors the growth of microorganisms. This study investigates the presence of yeasts in water samples collected from Diluvio Stream and assesses virulence factors and the susceptibility of antifungal agents commonly prescribed for the treatment of mycoses. Samples were collected at three sites, in two seasons of the year, seeded and isolated in different culture media supplemented with chloramphenicol. Biochemical assays, thermotolerance at 37oC, proteinase, and phospholipase activities were carried out to identify isolates. Tests to determine resistance to antifungal included a susceptibility test and minimum inhibitory concentration (MIC) evaluation using different antifungal agents. R...
Screening of Actinomycetes with Activity Against Clinical Isolates of Gram Positive Cocci with Multiresistant Profile
Actinomycetes are Gram-positive bacteria widely distributed in the environment and known for the ... more Actinomycetes are Gram-positive bacteria widely distributed in the environment and known for the diverse biologically act ive molecules they produce. This study assesses the activity of compounds produced by 40 actinomycete isolates against Enterococcus spp. Staphylococcus aureus and Staphylococcus epidermidis clinical isoalates. The susceptibility profile of the samples was assessed using the agar disk diffusion method. Antimicrobial activity of actinomycetes was evaluated according to the double layer method. Here, two isolates (50 and 8S) exhibited activity against 90% of clinical Staphylococcus spp. and Enterococcus spp. These isolates were grown in starch casein broth at 30˚C for 7 days with constant shaking. After, the culture obtained was filtered to obtain a crude extract, whose antibiotic activity was assessed using the well diffusion technique. In this assay, isolate 50 presented higher activities than that exhibited by isolate 8S. The isolate 50 has been used in different...
Uploads
Papers by Sueli Van Der Sand